PyBOP CAS 128625-52-5

Chemical Formula: C18H28F6N6OP2
Molecular Weight: Approximately 482.33 g/mol
Structure: PyBOP is a phosphonium salt and is often used in peptide synthesis as a coupling reagent, facilitating the formation of peptide bonds.
Appearance: It is typically a white to off-white crystalline powder.
Solubility: It is soluble in common organic solvents such as dichloromethane (DCM), dimethylformamide (DMF), and dimethyl sulfoxide (DMSO).

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PyBOP Technical Information:

Chemical Name

Benzotriazole-1-yl-oxytripyrrolidinophosphonium hexafluorophosphate

CAS Number


Common Name




Melting point

154-156 °C (dec.)(lit.)


1.438 at 20℃

Vapor pressure

0-0Pa at 20-50℃

Storage temp.



methanol: 25 mg/mL, clear


Fine Crystalline Powder


White to off-white


Moisture Sensitive



PyBOP (benzotriazol-1-yloxytripyrrolidinophosphonium hexafluorophosphate) is a peptide coupling reagent employed in solid phase peptide synthesis, serving as an alternative to the BOP reagent. Due to its efficacy and low racemization rates, PyBOP is extensively utilized in peptide chemistry to facilitate amide bond formation. Its action induces a nucleophilic attack by amines on carboxylic acids, which initiates peptide bond formation. It has advantages over some other coupling reagents in terms of minimal side reactions and ease of handling.


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